The constructed Escherichia coli efficiently adsorbed fenitrothion through the displaying a pesticide-binding peptide on it using the anchoring motif OmpC. Fenitrothion, or O, O-dimethyl O-(3-methyl-4-nitrophenyl) phosphorothioate, is a nitrophenolic pesticide that was widely used in agriculture. The inhibition of acetylcholine esterase activity and inhibition of acetylcholine breakdown in synapses result in the accumulation of acetylcholine in synapses, which causes convulsions, paralysis, and death of insects. However, under aerobic conditions, the major hydrolysis metabolite of fenitrothion, 3-methyl-4-nitrophenol, is toxic to many living organisms, raising environmental safety concerns. In this study, fenitrothion-adsorbing recombinant Escherichia coli was constructed through the display of pesticide-binding peptide, using OmpC as an anchoring motif.